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Drug Testing Services for DOT Regulated Companies

Our expansive national network of over 10,000 collection sites makes scheduling drug tests easy and saves your drivers time!
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DOT Drug Testing

Alliance 2020 works with companies required by the DOT to have a drug testing program in place. No matter which DOT agency your business is regulated by, Alliance 2020 can help.

Our collection network is highly expansive, consisting of 10,000+ sites. This means your drivers, pilots or other DOT covered employees will be able to easily find a collection facility. Through us you can schedule pre-employment, random, post accident, return to duty, follow up, and reasonable suspicion drug screenings easily through our electronic ordering and result reporting system.

In addition to DOT drug testing, Alliance 2020 can also handle DOT physical exam scheduling for your DOT covered employees. Additionally, we offer a wide range of other occupational health services.

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Reasons for DOT Drug Testing

There are five reasons why an employer may request a DOT drug test:


  • Pre-Employment
  • Random
  • Post-Accident
  • Reasonable Suspicion
  • Return to Duty

Substances Tested in DOT Urine Tests

A DOT drug test screens for the following classes of drugs:

  • Amphetamines and Methamphetamines
  • Marijuana (THC)
  • Opiates – Opium and Codeine Derivatives
  • Cocaine
  • Phencyclidine (PCP)

DOT drug testing rules specify confirmatory testing for any non-negative screens. These follow-up drug tests are required by the DOT to be observed.

Drug Testing

Prescriptions & Habit Forming Drugs

Individuals who drive commercial motor vehicles may not use habit-forming drugs, narcotics or amphetamines.

However, if a driver is prescribed one of these substances by a licensed medical practitioner, it may be allowed.

The practitioner must be familiar with the driver’s medical history and confirm that the prescribed drug will not adversely affect the driver’s ability to safely operate the vehicle.

Failed Tests & Refusals

If an applicant for employment refuses a drug test or tests positive, they are not permitted to perform safety-sensitive duties. Existing employees who test positive (or refuse a test) may not return to employment that entails safety-sensitive duties until they have seen a substance abuse professional (SAP) and successfully complete return-to-duty drug testing.

If a test comes back positive, we will specify the specific drug metabolites being used. Employers must report the number of verified positive drug tests on their annual MIS reports upon DOT request.

Third Party Administration (TPA) Services

As your third-party administrator, Alliance 2020 will review your current random management program and implement changes to streamline it.

It is important that your policies and procedures are efficient and in compliance with DOT regulations. A properly managed drug-testing program reduces liability for both your company and employees. We can provide a complimentary audit of your current drug testing program and identify areas of improvement.

Alliance 2020 offers significant advantages over other TPAs. We have an expansive network of over 10,000 collection sites, allowing for easier scheduling. Our customer service support team is based in Washington and always available to answer questions. Alliance 2020 is a partner you can trust to manage your random drug testing program.

DOT Drug & Alcohol Programs

Alliance 2020 Programs

Alliance 2020 are professionals at establishing and administering DOT-compliant testing programs for any business in the transportation industry. If you are a DOT-regulated company required to have a DOT-compliant drug and alcohol program in place, we can provide your business with complete testing and program services.

We strictly adhere to DOT compliance standards in all of our testing, which means when you use you can rest assured that your program will pass a DOT audit anytime. Unlike other medical facilities that simply offer drug testing, we are qualified to offer DOT-compliant testing with all the required components.

Alliance 2020 is here to ensure your company, its employees, and your drug and alcohol program stay in compliance at all times, so you can focus on your business.

Occupational Health Services

Alliance 2020 provides DOT physical exam scheduling as well as other occupational health services for your drivers. To learn more click here.

DOT-Drug Testing (Part 40)
TPA Services including random selection and reporting are provided at no additional charge when you participate in our drug testing program.
Designated Employee Rep. Training
Alliance 2020 can provide Designated Employee Representative ( DER) training for all transportation modes. Our training personnel is certified urine collectors and breathe alcohol testers ( BAT). It is suggested that at least two representatives be trained for this required function. Training can be held at your location.
Supervisor Training
Alliance 2020 can provide supervisor training for all DOT modes. Each supervisor of personnel must participate in a mandatory drug and alcohol training session. The purpose of the training is to orient the supervisor on the requirement s of the Department of Transportation regulations. Supervisors of safety-sensitive personnel who have not been trained are out of compliance with federal requirements Training can be held at your location.
Drug Testing Resources

Learn more about DOT Drug Testing specific regulations and the services we offer that follow these guidelines.

Frequently Asked Questions

Do motor carriers need to report their drug and alcohol results to FMCSA every year?

No, Drug and alcohol are only required to be provided FMCSA upon FMCSA request. Every motor carrier shall prepare and maintain their previous year’s drug and alcohol MIS report in a safe and secure location, with controlled access, and make them available for inspection when requested by authorized personnel. These records should be maintained for a period of 5 years. See 49 CFR 382.403 for more information. Every year FMCSA randomly selects a group of motor carriers to report their MIS results.

If you are selected, you will receive a notice with a username and password to file your previous year’s MIS drug and alcohol results online. If you are notified by FMCSA to report your previous year’s MIS results, you are required to formally submit your MIS drug and alcohol results to FMCSA. See §382.403 reporting of alcohol and controlled substances testing program results in management information systems. FMCSA expects a 100% response rate from motor carriers selected to report their previous year’s drug and alcohol testing results Motor carriers that don’t respond to the notice requesting submission of MIS results may be subject to civil penalties of up $1,000 dollars per day that the motor carrier fails to comply.

What is a Custody and Control Form (CCF) and do I need one?

Under the Substance Abuse and Mental Health Services Administration (SAMHSA), the Custody and Control Form (CCF) is the documentation required for drug test ordering, specimen collection processing, and chain of custody documentation for workplace drug testing.

CCFs, whether in a paper or electronic format, document the chain of custody during DOT-regulated drug tests. Chain of Custody Forms show a trail of the seizure, custody, control, transfer, analysis, and disposition of evidence of a human specimen test.

Explore additional information and resources regarding the role of CCFs for DOT-regulated companies here. Or, view/download the most current CCF here.

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