DOT Drug Testing Services
Alliance 2020 works with companies required by the DOT to have a drug testing program in place. No matter which DOT agency your business is regulated by, Alliance 2020 can help.
We have a highly expansive collection network consisting of over 10,000 sites. This allows your drivers, pilots or other DOT covered employees to conveniently get tested. Through us you can easily schedule drug screenings through our electronic ordering and result reporting system.
In addition to DOT drug testing, we also offer a full-range of occupational health services and training resources to our clients.

Streamlined Process
Clearinghouse Support
For FMCSA regulated clients, we can provide assistance with the clearinghouse. This includes conducting queries and reporting violations on your behalf. Your account manager will also provide you with guidance as needed.
Training Materials
We provide numerous training resources for our clients. This includes Designated Employer Representative (DER) and Reasonable Suspicion Training for Supervisors. Click here to learn more!
Designated Account Representative
Occupational Health Services
We provide a wide range of occupational health services to support your drug screening program, including DOT physical exam services. Click here to learn more!
Onsite Collection Services
Random Drug Testing Management Services
Alliance 2020 are professionals at establishing and administering DOT-compliant testing programs for any business in the transportation industry. If you are a DOT-regulated company required to have a DOT-compliant drug and alcohol program in place, we can provide your business with complete testing and program services.
We strictly adhere to DOT compliance standards in all of our testing, which means when you use you can rest assured that your program will pass a DOT audit anytime. Unlike other medical facilities that simply offer drug testing, we are qualified to offer DOT-compliant testing with all the required components.
We are here to ensure your company, your employees, and your drug and alcohol testing program stay in compliance at all times, so you can focus on your business.

Frequently Asked Questions
What are the reasons for conducting a DOT drug test?
There are five reasons why an employer may request a DOT drug test:
- Pre-Employment
- Random
- Post-Accident
- Reasonable Suspicion
- Return to Duty
What substances are tested for in DOT urine tests?
A DOT drug test screens for the following classes of drugs:
- Amphetamines (Amphetamine, Methamphetamine, MDMA, MDA)
- Marijuana (THC)
- Opioids (Codeine, Morphine, 6-AM (Heroin), Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone)
- Cocaine
- Phencyclidine (PCP)
DOT drug testing rules specify confirmatory testing for any non-negative screens.
What happens if my employee has a prescription for a prohibited substance?
Individuals who drive commercial motor vehicles may not use habit-forming drugs, narcotics or amphetamines.
However, if a driver is prescribed one of these substances by a licensed medical practitioner, it may be allowed.
The practitioner must be familiar with the driver’s medical history and confirm that the prescribed drug will not adversely affect the driver’s ability to safely operate the vehicle.
What happens if my employee refuses their drug test?
If an applicant for employment refuses a drug test or tests positive, they are not permitted to perform safety-sensitive duties. Existing employees who test positive (or refuse a test) may not return to employment that entails safety-sensitive duties until they have seen a substance abuse professional (SAP) and successfully complete return-to-duty drug testing.
If a test comes back positive, we will specify the specific drug metabolites being used. Employers must report the number of verified positive drug tests on their annual MIS reports upon DOT request.
How can I make sure my DOT drug testing program is in compliance?
Alliance 2020 can perform a DOT drug testing program audit if you’re unsure about your compliance status.
For those falling under FMCSA regulations, the FMCSA provides a Carrier Compliance Questionnaire to help check your compliance status. Click here to view this checklist.