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Drug Testing Services for DOT Regulated Companies

Our expansive national network of over 10,000 collection sites makes scheduling drug tests easy and saves your drivers time!
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DOT Drug Testing

Alliance 2020 works with companies required by the DOT to have a drug testing program in place. No matter which agency of the DOT your business is regulated by, Alliance 2020 can help.

Our collection network is highly expansive, consisting of 10,000+ sites. This means your drivers, pilots or other employees will be able to easily find a collection facility.

In addition to DOT drug testing, Alliance 2020 can also handle DOT physical exam scheduling for your drivers. Additionally, we offer a wide range of occupational health services.

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Part 40 Regulations

 49 CFR Part 40 is a DOT-wide regulation that states how to conduct DOT Drug Testing. It also explains the process of returning employees to safety-sensitive duties after they violate a DOT drug and alcohol regulation. Part 40 applies to all DOT-regulated testing, regardless of what agency-specific rule applies to an employer.

Whether you are an airline covered by FAA rules or a trucking company covered by FMCSA rules, Part 40 procedures for collecting and testing specimens and reporting of test results apply to you. Each DOT agency-specific regulation spells out who is subject to testing, when and in what situations for a particular transportation industry.

Reasons for DOT Drug Testing

There are five reasons why an employer may request a DOT drug test:


  • Pre-Employment
  • Random
  • Post-Accident
  • Reasonable Suspicion
  • Return to Duty

Substances Tested in DOT Urine Tests

A DOT drug test screens for the following classes of drugs:

  • Amphetamines and Methamphetamines
  • Marijuana (THC)
  • Opiates – Opium and Codeine Derivatives
  • Cocaine
  • Phencyclidine (PCP)

DOT drug testing rules specify confirmatory testing for any non-negative screens. These follow-up drug tests are required by the DOT to be observed.

Drug Testing

Prescriptions & Habit Forming Drugs

Individuals who drive commercial motor vehicles may not use habit-forming drugs, narcotics or amphetamines.

However, if a driver is prescribed one of these substances by a licensed medical practitioner, it may be allowed.

The practitioner must be familiar with the driver’s medical history and confirm that the prescribed drug will not adversely affect the driver’s ability to safely operate the vehicle.

Failed Tests & Refusals

If an applicant for employment refuses a drug test or tests positive, they are not permitted to perform safety-sensitive duties. Existing employees who test positive (or refuse a test) may not return to employment that entails safety-sensitive duties until they have seen a substance abuse professional (SAP) and successfully complete return-to-duty drug testing.

If a test comes back positive, we will specify the specific drug metabolites being used. Employers must report the number of verified positive drug tests on their annual MIS reports upon DOT request.

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